NMR- and MS-Based Untargeted Metabolomic Study of Stool and Serum Samples from Patients with Anorexia Nervosa

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dc.contributor.authorTomášová, Petra
dc.contributor.authorProcházková, Petra
dc.contributor.authorRoubalová, Radka
dc.contributor.authorDvořák, Jiří
dc.contributor.authorTlaskalová-Hogenová, Helena
dc.contributor.authorČermáková, Martina
dc.contributor.authorPelantová, Helena
dc.contributor.authorŠedivá, Blanka
dc.contributor.authorVecka, Martin
dc.contributor.authorPapežová, Hana
dc.contributor.authorKuzma, Marek
dc.date.accessioned2023-02-20T11:00:27Z
dc.date.available2023-02-20T11:00:27Z
dc.date.issued2022
dc.description.abstract-translatedAnorexia nervosa (AN), a pathological restriction of food intake, leads to metabolic dysregulation. We conducted a metabolomics study to reveal changes caused by AN and the effect of hospital realimentation on metabolism. Both stool and serum from patients with AN and healthy controls were analyzed by NMR and MS. Statistical analysis revealed several altered biochemical and anthropometric parameters and 50 changed metabolites, including phospholipids, acylcarnitines, amino acids, derivatives of nicotinic acid, nucleotides, and energy metabolism intermediates. Biochemical and anthropometric parameters were correlated with metabolomic data. Metabolic changes in patients with AN described in our study imply serious system disruption defects, such as the development of inflammation and oxidative stress, changed free thyroxine (fT4) and thyroid-stimulating hormone (TSH) levels, a deficit of vitamins, muscle mass breakdown, and a decrease in ketone bodies as an important source of energy for the brain and heart. Furthermore, our data indicate only a very slight improvement after treatment. However, correlations of metabolomic results with body weight, interleukin 6, tumor necrosis factor α, fT4, and TSH might entail better prognoses and treatment effectiveness in patients with better system parameter status. Data sets are deposited in MassIVE: MSV000087713, DOI: 10.25345/C57R7X.en
dc.format10 s.cs
dc.format.mimetypeapplication/pdf
dc.identifier.citationTOMÁŠOVÁ, P. PROCHÁZKOVÁ, P. ROUBALOVÁ, R. DVOŘÁK, J. TLASKALOVÁ-HOGENOVÁ, H. ČERMÁKOVÁ, M. PELANTOVÁ, H. ŠEDIVÁ, B. VECKA, M. PAPEŽOVÁ, H. KUZMA, M. NMR- and MS-Based Untargeted Metabolomic Study of Stool and Serum Samples from Patients with Anorexia Nervosa. JOURNAL OF PROTEOME RESEARCH, 2022, roč. 21, č. 3, s. 778-787. ISSN: 1535-3893cs
dc.identifier.document-number773706400021
dc.identifier.doi10.1021/acs.jproteome.1c00537
dc.identifier.issn1535-3893
dc.identifier.obd43937914
dc.identifier.uri2-s2.0-85117137717
dc.identifier.urihttp://hdl.handle.net/11025/51546
dc.language.isoenen
dc.publisherAmerican Chemical Societyen
dc.relation.ispartofseriesJournal Of Proteome Researchen
dc.rightsPlný text není přístupný.cs
dc.rights© American Chemical Societyen
dc.rights.accessclosedAccessen
dc.subject.translatedanorexia nervosaen
dc.subject.translatedmetabolomicsen
dc.subject.translatedmass spectrometryen
dc.subject.translatednuclear magnetic resonanceen
dc.titleNMR- and MS-Based Untargeted Metabolomic Study of Stool and Serum Samples from Patients with Anorexia Nervosaen
dc.typečlánekcs
dc.typearticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen

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