Chronic DDE Exposure Modifies Mitochondrial Respiration during Differentiation of Human Adipose-Derived Mesenchymal Stem Cells into Mature Adipocytes

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dc.contributor.authorKladnická, Iva
dc.contributor.authorČedíková, Miroslava
dc.contributor.authorJedlička, Jan
dc.contributor.authorKohoutová, Michaela
dc.contributor.authorMüller, Luděk
dc.contributor.authorPlavinová, Iveta
dc.contributor.authorKripnerová, Michaela
dc.contributor.authorBludovská, Monika
dc.contributor.authorKuncová, Jitka
dc.contributor.authorMüllerová, Dana
dc.date.accessioned2021-11-01T11:00:34Z
dc.date.available2021-11-01T11:00:34Z
dc.date.issued2021
dc.description.abstract-translatedThe contribution of environmental pollutants to the obesity pandemic is still not yet fully recognized. Elucidating possible cellular and molecular mechanisms of their effects is of high impor- tance. Our study aimed to evaluate the effect of chronic, 21-day-long, 2,2-bis (4-chlorophenyl)-1,1- dichlorethylenedichlorodiphenyldichloroethylene (p,p’-DDE) exposure of human adipose-derived mesenchymal stem cells committed to adipogenesis on mitochondrial oxygen consumption on days 4, 10, and 21. In addition, the mitochondrial membrane potential (MMP), the quality of the mito- chondrial network, and lipid accumulation in maturing cells were evaluated. Compared to control differentiating adipocytes, exposure to p,p’-DDE at 1 µ M concentration significantly increased basal (routine) mitochondrial respiration, ATP-linked oxygen consumption and MMP of intact cells on day 21 of adipogenesis. In contrast, higher pollutant concentration seemed to slow down the gradual increase in ATP-linked oxygen consumption typical for normal adipogenesis. Organochlorine p,p’-DDE did not alter citrate synthase activity. In conclusion, in vitro 1 µ M p,p’-DDE corresponding to human exposure is able to increase the mitochondrial respiration per individual mitochondrion at the end of adipocyte maturation. Our data reveal that long-lasting exposure to p,p’-DDE could interfere with the metabolic programming of mature adipocytes.en
dc.format15 s.cs
dc.format.mimetypeapplication/pdf
dc.identifier.citationKLADNICKÁ, I. ČEDÍKOVÁ, M. JEDLIČKA, J. KOHOUTOVÁ, M. MÜLLER, L. PLAVINOVÁ, I. KRIPNEROVÁ, M. BLUDOVSKÁ, M. KUNCOVÁ, J. MÜLLEROVÁ, D. Chronic DDE Exposure Modifies Mitochondrial Respiration during Differentiation of Human Adipose-Derived Mesenchymal Stem Cells into Mature Adipocytes. Biomolecules, 2021, roč. 11, č. 8, s. 1-15. ISSN: 2218-273Xcs
dc.identifier.document-number688852000001
dc.identifier.doi10.3390/biom11081068
dc.identifier.issn2218-273X
dc.identifier.obd43933254
dc.identifier.uri2-s2.0-85110463593
dc.identifier.urihttp://hdl.handle.net/11025/45616
dc.language.isoenen
dc.project.IDEF17_048/0007267/InteCom: VaV inteligentních komponent pokročilých technologií pro plzeňskou metropolitní oblastcs
dc.publisherMDPIen
dc.relation.ispartofseriesBiomoleculesen
dc.rights.accessopenAccessen
dc.subject.translatedhuman adipose-derived mesenchymal stem cellsen
dc.subject.translatedadipogenesisen
dc.subject.translatedp,p’-DDEen
dc.subject.translatedmitochondrial respirationen
dc.titleChronic DDE Exposure Modifies Mitochondrial Respiration during Differentiation of Human Adipose-Derived Mesenchymal Stem Cells into Mature Adipocytesen
dc.typečlánekcs
dc.typearticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen

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