New planar light source for the induction and monitoring of photodynamic processes in vitro
| dc.contributor.author | Bajgar, Robert | |
| dc.contributor.author | Pola, Martin | |
| dc.contributor.author | Hošík, Jakub | |
| dc.contributor.author | Turjanica, Pavel | |
| dc.contributor.author | Čengery, Jiří | |
| dc.contributor.author | Kolářová, Hana | |
| dc.date.accessioned | 2020-06-22T10:00:17Z | |
| dc.date.available | 2020-06-22T10:00:17Z | |
| dc.date.issued | 2020 | |
| dc.description.abstract-translated | We recently developed a new light source that allows for the continuous monitoring of light-induced changes using common spectrophotometric devices adapted for microplate analyses. This source was designed primarily to induce photodynamic processes in cell models. Modern light components, such as LED chips, were used to improve the irradiance homogeneity. In addition, this source forms a small hermetic chamber and thus allows for the regulation of the surrounding atmosphere, which plays a significant role in these light-dependent reactions. The efficacy of the new light source was proven via kinetic measurements of reactive oxygen species generated during the photodynamic reaction of chloroaluminium phthalocyanine disulfonate (ClAlPcS2) in three cell lines: human melanoma cells (G361), human breast adenocarcinoma cells (MCF7), and human fibroblasts (BJ). | en |
| dc.format | 11 s. | cs |
| dc.format.mimetype | application/pdf | |
| dc.identifier.citation | BAJGAR, R. ., POLA, M. ., HOŠÍK, J. ., TURJANICA, P. ., ČENGERY, J. ., KOLÁŘOVÁ, H. . New planar light source for the induction and monitoring of photodynamic processes in vitro. Journal of biological physics, 2020, roč. 46, č. 1, s. 121-131. ISSN 0092-0606. | en |
| dc.identifier.document-number | 521739400006 | |
| dc.identifier.doi | 10.1007/s10867-020-09544-7 | |
| dc.identifier.issn | 0092-0606 | |
| dc.identifier.obd | 43929647 | |
| dc.identifier.uri | 2-s2.0-85082044200 | |
| dc.identifier.uri | http://hdl.handle.net/11025/37041 | |
| dc.language.iso | en | en |
| dc.publisher | Springer | en |
| dc.relation.ispartofseries | Journal Of Biological Physics | en |
| dc.rights | Plný text je přístupný v rámci univerzity přihlášeným uživatelům. | cs |
| dc.rights | © Springer | en |
| dc.rights.access | restrictedAccess | en |
| dc.subject.translated | light source | en |
| dc.subject.translated | photodynamic effect | en |
| dc.subject.translated | in vitro | en |
| dc.subject.translated | microplate reader | en |
| dc.title | New planar light source for the induction and monitoring of photodynamic processes in vitro | en |
| dc.type | článek | cs |
| dc.type | article | en |
| dc.type.status | Peer-reviewed | en |
| dc.type.version | publishedVersion | en |