Hydrolytic instability of laser-ablatively deposited CaSi2 coatings in air and neutral water affects the behavior of bone healing-related cell types

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dc.contributor.authorKřenek, Tomáš
dc.contributor.authorPola, Josef
dc.contributor.authorStich, Theresia
dc.contributor.authorPattappa, Girish
dc.contributor.authorDocheva, Denitsa
dc.contributor.authorKoštejn, Martin
dc.contributor.authorMedlín, Rostislav
dc.contributor.authorMykisek, Petr
dc.contributor.authorJandová, Věra
dc.contributor.authorPola, Michal
dc.contributor.authorKovářík, Tomáš
dc.contributor.authorCassel, Júlia
dc.contributor.authorHolzman, Martin
dc.contributor.authorMoskal, Denys
dc.date.accessioned2025-05-19T18:53:36Z
dc.date.available2025-05-19T18:53:36Z
dc.date.issued2024
dc.description.abstractNestabilita silicidu vápenatého (CaSi2) ve vlhkém vzduchu nebo pH-neutrální vodě zůstává neznámá, ačkoli topochemická tvorba silicenu z CaSi2 v různých vodných fázích je dobře známý proces. Zde referujeme o laserové ablaci CaSi2 ve vakuu a etanolu, charakterizujeme povlaky nanesené na titanových substrátech pomocí elektronové mikroskopie, rentgenové fotoelektronové, Ramanovy a infračervené spektroskopie a zkoumáme nestabilitu nanesených povlaků ve vlhkém vzduchu a neutrální vodě. Dále zkoumáme chování lidských mezenchymálních stromálních buněk (hMSC), vaskulárních buněk (HUVECS, endoteliální buňky lidské pupečníkové žíly) a makrofágů (odvozených z buněčné linie THP-1) v kontaktu s deponovanými povlaky ponořenými do buněčného kultivačního média. Pozorované výsledky naznačují, že povlaky CaSi2 podléhají topochemické přeměně na silicen, která je doprovázena hydrolytickými reakcemi vedoucími k anorganickým sloučeninám Ca a SiO2, a že odezva všech typů buněk v hydrolyzované povrchové doméně CaSi2 je negativně ovlivněna povahou hydrolytické produkty. Na rozdíl od toho buňky vykazovaly tendenci ke zvýšené biokompatibilitě vůči částicím CaSi2 odstraněným ve vakuu. Výsledky naznačují, že postupy potahování mohou významně ovlivnit výsledky chování buněk.cs
dc.description.abstract-translatedCalcium silicide (CaSi2) instability in humid air or pH-neutral water remains unknown, although the topochemical formation of silicene from CaSi2 in various aqueous phases is a well-known process. Here we report on laser ablation of CaSi2 in the vacuum and ethanol, characterize coatinCalcium silicide (CaSi2) instability in humid air or pH-neutral water remains unknown, although the topochemical formation of silicene from CaSi2 in various aqueous phases is a well-known process. Here we report on laser ablation of CaSi2 in the vacuum and ethanol, characterize coatings deposited on titanium substrates with electron microscopy, X-ray photoelectron, Raman, and infrared spectroscopy, and examine the instability of the deposited coatings in humid air and neutral water. We further investigate the behavior of human mesenchymal stromal cells (hMSCs), vascular cells (HUVECS, human umbilical vein endothelial cells), and macrophages (derived from THP-1 cell line) in contact with the deposited coatings submerged in cell culture medium. The observed results indicate that the CaSi2 coatings undergo topochemical conversion to silicene, which is accompanied by hydrolytic reactions leading to inorganic Ca compounds and SiO2, and that the response of all cell types in the hydrolyzed CaSi2 surface domain is negatively affected by the nature of the hydrolytic products. In contrast, cells showed a tendency for enhanced biocompatibility towards the CaSi2 particles ablated in the vacuum. The results suggest that coating approaches can significantly influence cell behavior outcomes.gs deposited on titanium substrates with electron microscopy, X-ray photoelectron, Raman, and infrared spectroscopy, and examine the instability of the deposited coatings in humid air and neutral water. We further investigate the behavior of human mesenchymal stromal cells (hMSCs), vascular cells (HUVECS, human umbilical vein endothelial cells), and macrophages (derived from THP-1 cell line) in contact with the deposited coatings submerged in cell culture medium. The observed results indicate that the CaSi2 coatings undergo topochemical conversion to silicene, which is accompanied by hydrolytic reactions leading to inorganic Ca compounds and SiO2, and that the response of all cell types in the hydrolyzed CaSi2 surface domain is negatively affected by the nature of the hydrolytic products. In contrast, cells showed a tendency for enhanced biocompatibility towards the CaSi2 particles ablated in the vacuum. The results suggest that coating approaches can significantly influence cell behavior outcomes.en
dc.description.sponsorshipEH22_008/0004634 Strojní inženýrství biologických a bioinspirovaných systémůcs
dc.format18 s.cs
dc.identifier.doihttps://doi.org/10.1016/j.surfin.2024.105381
dc.identifier.urihttp://hdl.handle.net/11025/59176
dc.language.isoenen
dc.publisherElsevieren
dc.rights© CC BY 4.0en
dc.rights.accessopenAccessen
dc.subjectsilicid vápenatýcs
dc.subjectlaserová ablace ve vakuucs
dc.subjectlaserová ablace v kapaliněcs
dc.subjecthydrolýza CaSi2cs
dc.subjectbioaktivitacs
dc.subjectmezenchymální stromální buňkycs
dc.subjectHUVECcs
dc.subjectmakrofágycs
dc.subjectbiokompatibilitacs
dc.subject.translatedcalcium silicideen
dc.subject.translatedlaser ablation in a vacuumen
dc.subject.translatedlaser ablation in liquiden
dc.subject.translatedCaSi2 hydrolysisen
dc.subject.translatedbioactivityen
dc.subject.translatedmesenchymal stromal cellsen
dc.subject.translatedHUVECsen
dc.subject.translatedmacrophagesen
dc.subject.translatedbiokompatibilityen
dc.titleHydrolytic instability of laser-ablatively deposited CaSi2 coatings in air and neutral water affects the behavior of bone healing-related cell typesen
dc.typearticleen
dc.typečlánekcs
dc.type.statusPeer revieweden
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local.files.size18079387*
local.has.filesyes*

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