Automatic detection of neurons, astrocytes, and layers for NISSL-stained mouse cortex

Show simple item record
dc.contributor.authorNosova, Svetlana
dc.contributor.authorSnopova, Ludmila
dc.contributor.authorTurlapov, Vadim
dc.contributor.editorSkala, Václav
dc.date.accessioned2017-09-01T10:33:28Z
dc.date.available2017-09-01T10:33:28Z
dc.date.issued2017
dc.description.abstract-translatedWe present an image processing algorithm for automatic detection of cortex layers and cells from optical microscopy images for Nissl-stained mouse brain. For every layer of cortex we automatically detect a shape and localization of following cortex cells type: neurons of molecular layer, pyramidal neurons, stellate neurons, and astrocytes. The algorithm includes the steps of: preprocessing, neurons and astrocytes localization, neurons classification, refined cortex layer detection, neurons reclassification. For preprocessing we use converting to gray image, Gaussian blurring, converting to black-white image after background removing, and rough estimate of layers. We use morphological operations with variation radius of structure element for neurons localization and neurons classification.en
dc.format8 s.cs
dc.format.mimetypeapplication/pdf
dc.identifier.citationJournal of WSCG. 2017, vol. 25, no. 2, p. 143-150.en
dc.identifier.issn1213-6972 (print)
dc.identifier.issn1213-6980 (CD-ROM)
dc.identifier.issn1213-6964 (on-line)
dc.identifier.uriwscg.zcu.cz/WSCG2017/!_2017_Journal_WSCG-No-2.pdf
dc.identifier.urihttp://hdl.handle.net/11025/26292
dc.language.isoenen
dc.publisherVáclav Skala - UNION Agencycs
dc.relation.ispartofseriesJournal of WSCGen
dc.rights© Václav Skala - UNION Agencycs
dc.rights.accessopenAccessen
dc.subjectzpracování obrazucs
dc.subjectautomatická detekcecs
dc.subjectoptická mikroskopiecs
dc.subjectNISSL-barvenícs
dc.subjectmyší mozková kůracs
dc.subjectvrstvy kůrycs
dc.subjectneuroncs
dc.subjectastrocytcs
dc.subjectpředběžné zpracovánícs
dc.subjectmorfologické rysycs
dc.subject.translatedimage processingen
dc.subject.translatedautomatic detectionen
dc.subject.translatedoptical microscopyen
dc.subject.translatedNISSL-stainingen
dc.subject.translatedmouse cortexen
dc.subject.translatedcortex layersen
dc.subject.translatedneuronen
dc.subject.translatedastrocyteen
dc.subject.translatedpreprocessingen
dc.subject.translatedmorphology featuresen
dc.titleAutomatic detection of neurons, astrocytes, and layers for NISSL-stained mouse cortexen
dc.typečlánekcs
dc.typearticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen

Files

Original bundle
Showing 1 - 1 out of 1 results
No Thumbnail Available
Name:
Nosova.pdf
Size:
2.06 MB
Format:
Adobe Portable Document Format
Description:
Plný text
Download
License bundle
Showing 1 - 1 out of 1 results
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Volume 25, Number 2 (2017)