Micropatterning and Nanodropletting of Titanium by Shifted Surface Laser Texturing Significantly Enhances In Vitro Osteogenesis of Healthy and Osteoporotic Mesenchymal Stromal Cells

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dc.contributor.authorStich, Theresia
dc.contributor.authorAlagboso, Francisca
dc.contributor.authorPattappa, Girish
dc.contributor.authorChu, Jin
dc.contributor.authorMoskal, Denys
dc.contributor.authorPovolný, Michal
dc.contributor.authorSaller, Maximilian
dc.contributor.authorSchönitzer, Veronika
dc.contributor.authorScholz, Konstantin J.
dc.contributor.authorCieplik, Fabian
dc.contributor.authorAlt, Volker
dc.contributor.authorRudert, Maximilian
dc.contributor.authorKovářík, Tomáš
dc.contributor.authorKřenek, Tomáš
dc.contributor.authorDocheva, Denitsa Metodieva
dc.date.accessioned2026-04-17T08:53:43Z
dc.date.available2026-04-17T08:53:43Z
dc.date.issued2025
dc.description.abstract-translatedThe key to proper implant integration in bone replacement is to orchestrate the complex interactions between materials and tissues. Moreover, due to the rapid demographic shift towards aging societies and the increase in elderly and osteoporotic patients, it is of great importance that implant materials are osteointegrative in not only healthy but also compromised bone tissues. Here, titanium (Ti) scaffolds were subjected to shifted laser surface texturing (sLST) using a nanosecond pulsed laser to create an open pore macrotopography with micro-and nano-Ti droplets. In contrast to conventional laser texturing, which leads to high heat accumulation; in sLST, the frequency of laser pulses is low, allowing for resolidification, thereby creating a surface with abundant coverage micro-/nanodroplets. The main objective was to compare the cellular responses of human mesenchymal stromal cells (hMSCs) on sLST-textured Ti surfaces (LT-Ti) for the first time with standard sand-blasted, acid-etched surfaces (SLA-Ti). In-depth analyses of cell survival, proliferation, shape, mineralization, and gene expression were performed. Cell survival/proliferation was found to be similar on both surfaces; however, SEM imaging revealed differences in hMSC morphology. On LT-Ti, cells adopted well-rounded shapes, whereas on SLA-Ti they assumed more planar shapes. Bulk RNA sequencing performed after short-term culture on both surfaces disclosed expression changes in genes such as DUSP6, TNFSF12-TNFSF13 and SULT1A4. Remarkably, the osteogenic differentiation capacity of hMSCs was significantly enhanced on LT-Ti compared to SLA-Ti. Furthermore, aged/osteoporotic donor cohorts showed significantly enhanced matrix mineralization on LT-Ti. In conclusion, our novel results demonstrate that sLST-Ti surfaces are safe, highly biocompatible, can rescue patient-cohort-specific mineralization behavior, and therefore hold great potential for the development into next-generation implants, which are suitable for both the elderly and bone-compromised populations.en
dc.description.sponsorshipStrojní inženýrství biologických a bioinspirovaných systémů EH22_008/0004634cs
dc.format31 s.cs
dc.identifier.doihttps://doi.org/10.3390/jfb16110401
dc.identifier.issn2079-4983
dc.identifier.urihttp://hdl.handle.net/11025/67659
dc.language.isoen
dc.publisherMDPIen
dc.rightsCC BY 4.0.en
dc.rights.accessopenAccessen
dc.subjecttitanové lešenícs
dc.subjectlaserové texturování povrchucs
dc.subjectintegrace implantátůcs
dc.subjectmezenchymální stromální buňkycs
dc.subjectbuněčná odpověďcs
dc.subjectosteogenní diferenciace in vitrocs
dc.subjectosteoporózacs
dc.subject.translatedtitanium scaffoldsen
dc.subject.translatedlaser surface texturingen
dc.subject.translatedimplant integrationen
dc.subject.translatedmesenchymal stromal cellsen
dc.subject.translatedcell responseen
dc.subject.translatedin vitro osteogenic differentiationen
dc.subject.translatedosteoporosisen
dc.titleMicropatterning and Nanodropletting of Titanium by Shifted Surface Laser Texturing Significantly Enhances In Vitro Osteogenesis of Healthy and Osteoporotic Mesenchymal Stromal Cellsen
dc.typearticleen
dc.typečlánekcs
dc.type.statusPeer revieweden
dc.type.versionpublishedVersionen
local.files.count1*
local.files.size8720830*
local.has.filesyes*
local.identifier.eid2-s2.0-105023574793

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